Parasitology
Lab-2 (E.histolytica)
E.
histolytica
1- pathogenic parasite
2- trophzoite acute هذه االشياء هي سبب خطورة هذه المرحلة من الطفيلي
has :-1-pseudopodia for motile activity -2- centric karyosom and regular chromatin for
protolytic enzymes and toxic sub.
3-infective stage : cyst
4-diagnostic stage : chroniccyst acutetrophozoite
5-cyst and trophozoite diagnosis by : biopsy and stool examination and imaging study
6- diseases :
a. acute dysntric diarrhea
يوجد فقط تروفوزويت
b. chronic diarrhea ( perforation , ulceration of colon and peritoneal region)
يوجد تروفوزويت و سست
c. toxic colon
d. liver abscess
e. lung and brain (rare)
7- route of infection :: feco-oral route ( water-food-flies-carriers)
8- habitat : large intestine mainly in cecum
9- treatment : metronidazole - tinidazole
10- E.histolyic is epidemic because it infect only human
11- morphology :
Trophozoite:
ليس لديه شكل ثابت
1- Clear ectoplasm .
2- Large finger – like pseudopdia
3- The endoplasm is granular and may contain RBCs.
4- It has one nucleous, contain small central keryosome and fine chromatin
granules arrenged regularly beneath nuclear membrane.
5- size: 30-50 microns
Mature Cyst:
1- small , spherical in shape , containing 1-4 nuclei
.
Each nucleous contain similar
nuclear morphology like the trophozoite but smaller , usually found in feces .
2- size: 10 microns
Immature Cyst:
1- containing 1-2 nuclei
2- chromatoid bodies
3- glycogen
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5
: فحوصات وهي
1- history (clinical presentation)
2- imaging technology
3- histological examination
4- serological examination
5- stool , urine and CSF examination