Parasitology
Lab-3
(
Giardia lambilia)
1- reproduction : by binary fission ( in normal condition)
2- common in our society and it infect all ages ,, it make endemic diseases .
3- habitat : lumen of small intestine mainly in duodenum and in the biliary tree .
4- route of infection : feco-oral route
5- infective stage : cyst
6- diagnostic stage : tropozoite (-)+ cyst
7- morphology :
Trophozoite:
1- small –> 12-15 micron
2- tear or piriform in shape
3- anterior rounded end and posterior tapered or pointed end
4- two large anterior nuclei with central karyosome .
5- has 2 axostyle
6- has 3-5 anterior flagella with single posterior flagella
7- has plepharoplast(sucking disc) and two parabasal bodies
8- dorsal convex surface and ventral flat surface
All these features is called ((
ugly monkey face
))
Cyst:
1- small , oval in shape , containing 4 nuclei one pair on each side of the axostyle
2- size: 10-12 microns
3- has 2 axostyle
4- it contain the remaining parts of trophozoite
The disease of Giardia lambilia :
1- steatorrhea
2- gallstones ( sever case )
3- lactose tolerance ( rare case )
4- diarrhea
5- weight loss
Giardia lambilia is mild but in rare conditions it make (( post giardial infection chronic
fatigue syndrome )) which may lead to death
The diagnosis of Giardia lambilia :
1- most common is stool examination for trophozoite or cyst but most of cases is lead
to negative result with this examination
2- the best examination is by enterocapsule
3- endoscope
4- duodenal aspiration
The treatment of Giardia lambilia :
1- metronidazole
2- tinidazole
( Triechomonus )
1- T.vaginalis ( for human)
2- T.tonex ( in crural areas – make bad breathe odor – does not make infection)
3- T.hominis (nonpathogenic – but in large number in colon lead to diarrhea )
( T.vaginalis )
1-Morphology
( of trophozoite
)
1- undulating membrane
2- large single anterior nucleus
3- 18-20 microns
4- single posterior flagella with 3 anterior
5- has axostyle
6- has hydrogenosome
7- piriform shape
8- sometimes it contain viral particles (HIV)
2- no cyst stage ( only trophozoite )
3- in female it infect the vagina but in male it infect the urethra and prostate
4- in female it secret toxic substances that make hemorrhage and damage to the epth.
Of the vagina and make a lot of exudate
5- in male it make urgency and irritation of urethra and infertility and little exudate
6- Diagnosis of T.vaginalis
1- Swap from the wall of the vagina
2- Semen analysis
7- Infection route ::
1- sexual way ( portal of entry is genitalia )
2- close contact to the patient
3- toilet
4- clothes
8- diagnostic and infected stage is trophozoite
9- The treatment of T.vaginalis:
1- metronidazole
2- tinidazole