Parasitology
Lab-5 (Lishmania)
1-hemoflagellate ( lishmania and trypanosoma ) have Amastigote , promastigote ,
epimastigote and trypomastigote stages .
2- Lishmania :
-- L. Donovane. (kala azar, Visceral leishmaniasis)
-- L. Tropica. ( Oriental sore, Baghdad boil, cutanous leishmaniasis)
-- L. barazerliensis (cutanous leishmaniasis )
-- L . Mexicana ( cutanous leishmaniasis )
3- morphology of all Lishmania
1- Amastigote
:: intracellutlar – round(ovale) – 2 to 4 microns – has nucleus and
kinetoplast – short internal flagellat – not motile
2- promastigote
:: spindle - has nucleus and kinetoplast – short external flagellate –
motile – 15 to 25 microns
5- diagnostic stage is Amastigote
(( in skin or RES ))
6- infective stage is promastigote
(( in the insect or the culture ))
7- life cycle :: transfer from man as amastigote to the vector(insect) then developed
into promastigote then transfer to man again
8- vector is sand-fly ( phlebotomas )
9- reservoir is man
10 – route of infection ::: skin bite – blood transmission – congenital(rare)
11-
diagnosis
:: 1- smear from the margin of ulcer ( see amastigote )
2- incubation in experimental animals
3- culture ( nnn media)
4- biopsy from ulcer
5- serological test ( PCR – skin test - ……… )
12- treatment : antimonials ( sodium stibogluconate )
13- habitat : in the RES ( liver – spleen – L.N – B.M ) and in skin
مالحظ
ات
::
1
-
ال
amastigote
تكون صغيرة جدا جدا و تحتوي على
غشاء رفيع جدا يحيط بها و
بالوسط يوجد نقطتان هما النواة وال
ل
kinetoplast
2
-
ال
promastigote
تظهر احيانا على شكل وردة
rosette
3
-
اذا كانت ال
amastigote
موجودة
في ال
skin
فهذا يعني
L.Tropica
و اذا كانت
موجودة بال
spleen
فهذا يعني
other Lishmanias