Parasitology
Lab-6 (P.vivax)
1- Plasmodium has two stages : -1- Vertebrate host---asexual cycle---schizogony
-2- Invertebrate host---sexual cycle---sporogony
2- schizogony divided into two stages :
-1- exoerythrocytic stage (hepatic stage)( in the liver ) (( not see in this lab ))
-2- erythrocytic stage ( in the RBCs ) ((
see in this lab
))
3-
infective stage
is sporozoites which seen in the salivary gland of the insect only
4-
diagnostic stage
of p.vivax is ameboid stage
5- why we see large RBCs in p.vivax infection ???? because
-1- the merosoite enter inside the RBCs and cause swelling of them
- 2- vivax infect reticulocyte (large immature RBC)
6- the cycle ( )نفس النظري-------
vivaxmerozoiteenter the RBCring stage formation of schuffiners dots(use for
diagnosis of vivax)active cytoplasm movementameboid stageimmature
stage(nuclear division)mature stage(cytoplasmic division )formation of 12-16
merozoite repeat the cycle or lead to formation of gametocyte (female
macrogametocyte and male microgametocyte)
7- vivax cause
tertian fever
( every three day )
8- diagnosis by :
-1-thick blood film : to recognize the presence of parasite ( in lite infection
and in endemic area because it is easy and fast )
-2-thin blood film : to differentiate between species
-3-serodiagnosis
9- malaria
only
occur inside the cells (intracellular)
10- you can not see multiple un-sequential stages in the same slide
ال يمكن رؤية عدة مراحل غير متسلسلة في نفس الساليد
11- route of infection: bite of female anopheles mosquito - sometimes blood transfusion
12- habitat : liver – RBC
13- the stages :::>>>
-1-
ring stage
: one nucleus + schuffiners dots { pink or red color }
-2-
ameboid stage
: one nucleus + schuffiners dots + irregular cytoplasm
-3-
immature stage
: more than one nucleus + schuffiners dots + irregular cytoplasm
-4-
mature stage
: multiple nucleus with their cytoplasm + schuffiners dots
(Mature stage )غير مطلوب في هذا المختبر