
Parasitology
Lab-7 (P. malariae + P.falciparum)
1- P. malariae
1- route of infection :: bite of female mosquito
2- it has no hypnozoite but p.vivax has hypnozoite
3- has dark brown to black malarian pigments
4- gametocyte are rounded
5-
infective stage
is sporozoites
6-
diagnostic stage
of p.malariae is band stage and rosette mature schzonite stage
7- it infect mature RBCs and the size of RBC still normal
8- the cycle ( )نفس النظري-------
malariaemerozoiteenter the RBCring stage (no schuffiners dots)(small and
compact ring) band stage (diagnostic stage) immature stage(nuclear
division)mature stage(sometimes has rosette shape with malarian pigment in the
center and the merozoite around it) formation of 6-10 merozoite repeat the cycle
or lead to formation of gametocyte (female macrogametocyte with compact nucleus
and male microgametocyte with diffuse nucleus)
9- malariae cause
quatrain malaria
( every four day )
10- diagnosis by :
-1-thick blood film : to recognize the presence of parasite ( in lite infection
and in endemic area because it is easy and fast )
-2-thin blood film : to differentiate between species
-3-serodiagnosis
11- malaria
only
occur inside the cells (intracellular)
12- erythrocytic sch. Cycle in P. malariae take 72 hours but in P.vivax take 42 hours
13- exoerythrocytic sch. Cycle in P. malariae take 2 weeks but in P.vivax take 10 days

14- the stages :::>>>
-1-
ring stage
: small compact ring + no schuffiners dots + normal size RBCs
-2-
band stage
: diagnostic
-3-
mature stage
: 6-10 merozoite + sometimes rosette
2- P. falciparum
1- cause Malignant tertian + Subtertian malaria
2- infect all types of RBCs
3- sometimes multiple infection in single RBC
4- sometimes the ring stage has two nucleus
5- in the peripheral blood see the ring and gametocyte only but in sever state we can
see the other stages
6- gametocyte crescent in shape ( diagnostic )
7- ring stage very small
8- mature schizonte has 8-32 merozoite
9- the stages :::>>>
-1-
ring stage
: very small غير موجودة بالمختبر
-2-
gametocyte
: crescent shape