Parasitology
lab-8(sexual cycle of malaria)
1- definitive host :: female anopheles mosquito ---- the male feed on plants not
blood
2- intermediate host :: human
3- infective stage of all malaria :: sporozoite ( present in the saliva of female anopheles
mosquito )
4- the sexual cycle occur in the mosquito as follow ::: the mosquito bite the skin of
infected human and take all stages of malaria inside the blood but all these stages are
digested in the small intestine of the mosquito except the gametocyte which form the
zygote and after one hour the zygote form the ookenite then it go to the gut of the
mosquito to form the oocyst which go below the basement membrane of the gut and
increase in number and make nuclear division then rapture and release the sporozoite
which go to the body cavity then to the salivary gland then go to other human by bite
of the skin to repeat the asexual cycle in the human
5- sexual cycle ( sporogony ) in the female anopheles mosquito
6- asexual cycle ( schizogony ) in human
7- the slides :::
-1-
oocyst
:: 50 microns ,, spherical ,, in the intestine of mosquito
-2-
ookinete
:: small ,, motile(the zygote is immotile) ,, contain vacuoles ,, central
nucleus ,, anterior pointed end ,, posterior rounded end
-3-
sporozoite
:: elongated spindle shape ,, motile ,,central nucleus ,,10-15 micron
The differences between the sporozoite and the gametocyte of P. falciparum :::
Sporozoite gametocyte of P. falciparum
Larger smaller
Without RBCs with RBCs
Contain vacuoles no vacuoles
Central nucleus elongated nucleus
Note :: هام لإلمتحان
1- p.malariae ::: low degree of movement so it form band stage
2- p.vivax ::: high degree of movement so it form amoeboid stage
P.vivax Benign tertian malaria
P.malariae Quartan malaria
P.falciparum Malignant tertian malaria ( Subtertian malaria )
P.ovale ovale tertian malaria ( Benign tertian malaria )
Drugs used in treatment and prevention of malaria:
1. Quinine
2. Chloroquine
3. Sulfadoxine + pyrimethamine (fansidar)
4. Primaquine
5. Mefloquine
6. Proguanil