Q1:
1- Bismuth sulfate agar
2- Bismuth sulfate – brilliant green - Ferrous sulfate
3- Salmonella
Q2:
1- Lactose – bile salt – neutral red – crystal violet
2- mo.1 klebsella mo.2 E.coli
3- mucoid , large and aggregated colonies
Q3:
1- sugar utilization test
2- N.meningococci
3- Gram -ve - arranged in pairs – kidney shape – non motile – non spore forming
Q4:
1- Genus penicillium ( fungus )
2- Sabouraud agar // velvery texture – white color then blue green – rapid grower
3- Class Deutromycete
Q5:
1- Mycobacteria
2- Ziehl_Neelsen staining technique
3- 1.flood the fixed slide with carbol fuchsin & allow to steam genetly for 5 min (the stain should
not boil,so if it does reduce heat) ,if the stain appear to be evaporating &drying up too rapidly
,add alittle more.& keep the slide flooded,,,wash with water
2.apply sulfuric acid 20%.for 2 min ,,wash with water
3.apply ethyl alcohol _95% for 2 min ,,wash with water
4.apply the counter stain (methylene blue) for 30 sec,,wash with water. Blot ,allow to dry in air
& examine with the oil objective.
Q6:
1- T cell marker ( CD3 or CD4 )
2- T- cells ( CD3 – CD4 – CD8 ) // B- cells ( CD19 – CD20 – IgM – IgD ) // NK cells ( CD56 )
Dendritic cells ( CD 11c – CD123 ) // Stem cells ( CD34 )
Q7:
1- Pseudomonas
2- Convex colonies – 2-4mm in diameter – grape like odour
3- pyocyanin - pyoverdin - pyorubin - pyomelanin
4- _ _ _ + (( _ )) يعني فراغ
Q8:
1- Potassium tellurite media
2- Selective / Potassium tellurite and blood or serum
3- Genus corynebacterum
Q9:
1- Green –ve yellow +ve
2- Thiosulfate - citrate - bile salt- sucrose
3- G.vibrio // smooth – yellow – opaque center – transparent periphery
Q10:
1- PCR amplifies the NA enzymatically from patient sample ( use DNA polymerase enzyme )
2- Conventional end point analysis + qualitative // real time real time reading the result +
qualitative and quantitative
Q11:
1- Mancini test
2- 1.Gell is incorporated with an antibody against the antigen to be detected (e.g anti-x if we
want to detect x antigen)
2.Precipitation ring is resulted from the reaction between the Ab incorporated into gell and Ag
of the sample.
3. Square diameter of the ring is proportional to the concentration of Ag.
Q12:
1- mo.1 staph.epidermidis mo.2 staph.saprohyticus mo.3 staph.aureus
2- gram +ve - cocci - irregular clusters - non motile - non spore forming - spherical