عملي طفيليات 1 وورد

طفيليات عملي / ثالث طب

4 / 10 / 2016

Lab. 1 dr. Zainab K. Khalil
*Direct Wet Mount Preparation method:
All stool samples should be subjected to direct microscopic examination in order to detect the presence of protozoan trophozoites, cysts, and especially to illustrate trophozoites .
Method:
A drop of normal saline placed on the middle of a clean slide.
A small portion of stool sample mixed with the drop of saline by wooden stick.
The mixture covered with cover- slip.
The slide examined using light microscope.

***Iodine staining method:

This method mainly used to clarify morphological details of the protozoan parasite. Cyst can be specifically identified in this mount.
The procedure is carried out as following:
Wet smear of stool sample directly prepared by mixing drop of normal saline and stool on a clean microscopic slide.
A drop of iodine placed on the preparation, then covered with a cover- slip.
The slides examined with light microscope using different objective lens powers.


In the iodine mount, cytoplasm of the cysts stained yellow or light brown and nuclei stained dark brown.