د.ساجدة

WBC manual count using hemocytometer

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Introduction

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Material

Blood sample (EDTA anticoagulated blood or capillary blood) WBCs diluting pipette Diluting solution Hemocytometer microscope
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The Hemacytometer

contains 2 Neubauer counting chamber Each chamber contains: *4 WBC counting squares *Each contains 16 squares
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Methodology

draw blood up to 0.5 marks on WBCs pipette and complete to 11 with WBCs diluting solution(Turk‘s solution).Mix for 2-3 minute. *

Charge hemacytometer

Discard the first 4-5 drops Place tip of the pipette at the edge central platform Then let the hemacytometer to stand on the bench for 3-5 minute.
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Count and calculate:
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Turk's solution

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Calculation

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Calculation

The dilution factor= total volume/sample volume = 11-1/0.5 =20
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Calculation

counted volume = The total volume of the 1 large square= = (width x length x depth ) = (1mm x 1mm x 1/10 mm) =1/10
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Calculation

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Example:

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Normal values of WBCs:

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Significance of the test

Leukocytosis Occur as an indicator of body defense against foreign materials (bacteria, parasites, toxins) Metabolic disorders Chemical and drug poisoning Acute hemorrhage
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Significance of the test

Leukopenia Result from X-ray therapy Alcoholism Antibiotic therapy Typhoid infection Measls Infectious hepatitis TB And cirrhosis of the liver
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THANKS

Thanks
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