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Introduction
The triacylglycerols (TG) are esters of the trihydric alcohol glycerol and
fatty acids.
Triacylglycerols (Triglycerides) are the main storage forms of fatty
acids. Adipose tissue is the main store of triacylglycerol in the body.
Lipids are transported in the plasma as lipoproteins (96%) and free fatty
acids 4%(micelle + albumin/free fatty acid). There are four major lipid
classes are present in lipoproteins:
Triacylglycerols (16%)
Phospholipids (30%)
Cholesterol (14%)
Cholesteryl esters (36%).
Generalized structure of a plasma lipoprotein.
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Clinical significance
The measurement of the concentration in blood triglycerides is
important for the diagnosis and the follow up of hyperlipidemia. Its
increases can be of genetic origin or secondary to other metabolic
disorders such as
: Diabetes mellitus, hyper and hypotherodism, hepatic
diseases, pancreatitis and nephrosis.
Principle
The absorbance of the coloured complex quinonemine, propertional to
the amount of triglycerides in the serum, is measured at 500 nm.
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Procedure
Blank
Standard
Test
Reagent
1 ml
1 ml
1 ml
D.W
10
l
Standard
10
l
serum
10
l
let stand for 10 minutes at room temperature. Read absorbance at 500
nm against reagent blank.
Note:
Reagent= Buffer
+ Enzymes.
Standard= Triglycerides 200 mg
\dl or 2.28 mmol\L.
Calculation