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Systimatic bacteriology

D. Faten naeem abbas

• Gram positive cocci bacteria

• Genus Streptococcus
• Part:II

S. agalactiae

Beat hemolytic
Lancefield group B
They are members of the normal flora of the female genital tract vagina in 5-25% of woman and in urethral mucous membranes of male carriers as well as in the G.I.T.
It can transfer from infected mothers to infants at birth and may cause:
(1) meningitis and septicemia in neonates with high mortality rate,
(2) endocarditis
(3) septicemia or pneumonia in impaired immune systems individuals .
(4) diabetic foot infections.
Pathogenesis: Capsule, hemolysin and cAMP factor

• Lab. Identification :

Their colonies on blood agar are larger and less hemolysis than Group A
Based on their resistance to antibiotics it can be concluded that ampicillin in combination with gentamicin gentamicin/netilmicin is first choice course of therapy
Strep. group B resistant for bacitracin .
cAMP test-positive:
The CAMP factor produced by GBS acts synergistically with the staphylococcal β-hemolysin inducing enhanced hemolysis of sheep erythrocytes. Organism produces definitive arrow when close to Staphylococcus aureus.


Streptococcus Part II


Streptococcus Part II




Streptococcus Part II




Streptococcus Part II

Streptococcus pneumonia (pneumococcus)

This bacterium G +ve, diplococci
alpha hemolytic on blood agar,


Streptococcus Part II


Streptococcus Part II


Streptococcus Part II

Antigenic Structures

• It posses a number of Ags:
• 1- Peptidoglycan
• 2- Teichoic acid (Specific Carbohydrate)
• 3- M-protein
• 4- Polysaccharide capsule are used to determine the type specificity of organisms and virulence. so the pneumococcus can be serologically classified into more than 90 types
• The capsular polysaccharides (SSS) Typing can carried out by:
• 1- Agglutination of cocci with type – specific antisera (Ag + Ab reaction).
• 2- Precipitation of capsular polysaccharide with specific serum.
• 3- Capsular swelling reaction (Quellung reaction). Suspension of pneumococci is mixed with specific anti-polysaccharide serum of the same type or the polyvalent antiserum on slide, the capsule swells. This reaction useful for rapid identification and for typing of the microorganism (Figure 4).

Toxin and Virulence Factors

1- Pneumolysin O: an oxygen sensitive toxin, cytolytic for cells.
2- Neuraminidase: destroys surface structure of host tissues.
3- Capsular polysaccharide: It’s SSS which protects the microorganism from phagocytosis and has association with virulence.
4- C- substance: The cell wall of pneumococci contain teichoic acid (C-sub) that react with a certain antiserum protein, C- reactive protein (CRP) and causes activation of some nonspecific host immune response.
5- Leucocidin: Kills leukocytes W.B.C.

Pathogenesis

It’s a normal flora of nasopharynx and oropharynx in 15% of children and 5% of adults.
They produce disease through their ability to multiply in the tissues and the virulence of pneumococci is a function of it’s capsule which prevents ingestion by phagocytes.
The normal respiratory mucosa posses natural resistance to the pneuomococci, many factors lower this resistance and predispose to infection, like:
1- Respiratory viral infection, bronchial obstruction, respiratory tract
injury, alcohol or drug intoxication .
2- Organism passes from person to another via respiratory secretions and aerosols.

Clinical Findings

The onset of pneumococcal pneumonia is usually sudden with fever, chills and sharp pleural pain, with blood or rusty sputum. In the early disease, when the fever is high, bacteremia is present in about 10-20% of cases, causes severe complication as meningitis, which is the most serious infection common in children, endocarditis, septic arthritis.
1- Acute bacterial pneumonia
A leading causes of death in the aged and those whose resistance is impaired. This disease caused mostly by Strep. pneumonia. Pneumonia is preceded by an upper or middle respiratory viral infection, which predisposes to Strep. pneumonia infection of pulmonary parenchyma, by the mechanisms of:
• 1) increased volume and viscosity of secretions that are more difficult to clear,
• 2) Secondary, inhibition of the action of bronchial cilia by viral infection.

• 2- Otitis media

• Which is the most common bacterial infection of children, is most
• caused by pneumococcus, followed by Haemophils influenza and
• Moraxella catarrhalis.
• The traditional treatment of it with a Beta-lactam antibiotics (with or
• without a penicillinase-inhibitor) has been threatened by the spread of penicillin -resistant pneumococci.
• 3- Bacteremia/ sepsis
• In the absence of a focus of infection is commonly caused by pneumococcus, especially in splenectomized individuals.
• 4- Meningitis
• H. influenzae was leading cause of bacterial meningitis in U.S.
• After a vaccine was developed against this organism, Strep. Pneumonia became the most common cause, which is high mortality rate even when treated.

Laboratory Diagnosis: :

Specimens:
nasopharyngeal swab,
pus,
sputum
spinal fluid.
Microscopic examination :
• show many polymorphonuclear, neutrophiles and many RBC. direct smear stained by gram stain, show G+ve diplococci lancet–shaped, there is unstained halo around the organism which represent its capsule


Streptococcus Part II


Streptococcus Part II

• Macroscopic examination :

• Enriched media with blood or serum necessary for growth, produce alpha-hemolysin on blood agar growth is enhanced by 5-10% CO2.
• On solid media: form a small round colonies dome-shaped at first, few hours later it undergoes some autolysis in the center of the colony and become flat while the edges are raised.
Streptococcus Part II

• Biochemical tests :

• 1- Optochin sensitivity
• 2- Lysis the cell by bile acids
• 3- Capsular swelling (the Quellung reaction) is observed when sputum treated by type-specific antisera as immunological test.
• 4- Inuline fermentation to different from Sterp. viridans
• Animal pathogenicity :
• mice are most susceptible to pneumococcal infection and used for diagnosis, animal will die in 18-48h.
Streptococcus Part II

Optochin Susceptibility Test

Streptococcus Part II

Optochin susceptible

S. pneumoniae

Optochin resistant

S. viridans

Streptococcus viridans

Include species : Sterp. nitis , Sterp. mutans and Sterp. salivarins
Large group of commensal streptococci of the upper respiratory tract,
Either alpha-hemolytic producing a green coloration on blood agar (hence the name viridans in latin it is green )
They possess no Lancefield antigens
In general pathogenicity is low Pathology
The most abundant organism in mouth
One member of the group S. mutans is the etiologic agent of dental caries
Others may be envolved in other mouth or gingival infections
They are the most common causes of subacute bacterial endocarditis.

Infections of Viridans streptococi

1- S. mutans in combination with Lactobacilli the leading cause of dental carries .
2- Viridans S. (S. mutans, S. sanguis, S. salivarius, and S. mitis) are the most common cause of infective endocarditis.
3- Viridans S. ( S. milleri, and S. intermedius) causing brain abscesses often in combination with mouth anaerobes.
4- Viridans S. are involved in mixed aerobic-anaerobic infections in other areas of the body eg, abdominal abscesses

Strepto viridans

• Strepto. pneumonia

Test
Non capsulated
• Capsulated
• Microscopical morphology
oval or round arranged in chains
Flame shaped diplococci

Dome – shaped

Initially dome-shaped later flatted colonies
Culture colonies
• -

+
Quelling test
Granular turbidity and powdery deposits
Uniform turbidity
Growth in liquid media
not
soluble
solubility in bile salt
• -

+
Inulin fermentation
Resistant
Sensitive
Optochin sensitivity
• non pathogenic

Cause fatal infection

Intraperitoneal inoculation in mice
• Differentiation between Streptococcus pneumococcus and Streptococcus viridans

Group D Streptococci

(Enterococci and Non-enterococci)
Enterococcus (E.faecalis, E.faecium):
always present in colon. If it leaves its normal habitat (the colon), it can cause suppurative lesions, UTI, peritonitis, or puerperal sepsis.
It can grow on ordinary media and also on macConkey’s on which it gives deep pink colonies.
Multi-drug resistant, e.g. Vancomycin resistant enterococci (VRE) have developed and have been spreading.
Grow well in 40% bile and on 6.5% NaCl.
Hydrolyzes esculin in 40% bile and 6.5% NaCl (bile esculin agar turns black).
Varies Hemolysis
PYR +ve

Non-enterococcus

(Streptococcus bovis, Streptococcus equinus ):
Like the enterococci, Streptococcus bovis is hardy growing in 40% bile (but not in 6.5% NaCl).
It lives in the G.I. tract, and it causes similar diseases.
An important unique property is that there is a remarkable association between S. bovis infection and colon cancer!!! In some series 50% of people with S. bovis
bacteremia have a colonic malignancy. We do not know if S. bovis is a cause of colon cancer or just a marker of the disease.


رفعت المحاضرة من قبل: Ali Ahmed
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