Lab.4
3
rd
-year class
Practical Pharmacology
2010-2011
effect of Atropine on man
Procedure:-
1.Student are divided into 4 groups.
2.One student of each group acts as a volunteer.
3.The following
observations
are recorded 5
minutes interval prior to injection of Atropine.
a. Subjective symptoms.
b. Salivation.
c. Pulse rate.
d. Size of pupil.
e. Reaction to light.
f. Accommodation
N.B:-
to calculate the volume of saliva, we
collect saliva for 3 minutes after sucking a
drop of ascorbic acid(vitamin c).
4. Injection of Atropine solution
1ml(0.6
mg
) is
given
subcutaneously
to each volunteer.
5. The same observations are made after
injection, at 10 minutes interval for 40
minutes.
6. Make a table for the obtained data as
follows:-
Before injection after injection
40 min
30 min
20 min
10 min
5 min
Parameter
Subjective
Salivation
Pulse rate
Size of pupil
Reaction to light
Accommodation