micro by hassan almalah

عباره عن صور مختبر المايكرو من المحاضرات فقط جمعتها ووضعتها بهذا الملف
يحتوي على صور مختبرات فقط بعد نصف السنه
هنالك صور قليله مسحوبه من النت
مختبرات 13 و 14 أَخذت من العام الماضي لعم توفرها لدي
Done by me Hassan Almalah
Group – B -

Lab-12-

Staphylococci

Catalase tests

• (-) (+)

Coagulase test

Reaction to carbohydrate mannitol.

Dnase test

Novobiocin sensitivity test

Lab-13-

Streptococci


Streptococci

a-chains b- pairs (S. Pneumonia)

β-hemolytic Sterptococci.α-hemolytic Sterptococci.Non-hemolytic streptococci

β-hemolytic Sterptococci.


• It causes complete hemolysis to RBCs (caused by hemolysins) leading to formation of clear zone around the colonies
• Example: S. pyogenes (group A β-hemolytic Strept.), S.agalactiae

α-hemolytic Sterptococci.

• It causes:
• 1. Zone of greenish discolouration around the colonies.
• Example S.pneumonia ,s.viridans

Bacitracin sensitivity

• Bacitracin will inhibit the growth of gp A Strep. pyogenes giving zone of inhibition around the disk

CAMP test

Optochin Susceptibility Test

Bile Solubility test

Lab-14-

Sterptococcus Pneumoniae

bile solubility test

• Result :
• + ve clear
• - ve Turbidity
• Lyses of colonies means the microorganism become slouble in bile salt

Optochin disc test ( ethylhydro cupriene

• Result :
• If the inhibition zone a greater than 18mm that means its colony of St. pneumoniae while other streptococcus hemolytic hare zone less than 18mm

Quelling reaction ( capsular swelling reaction ) ( Serological test )

• Result :
• + ve capsule appear swollen
• - ve capsule quite invisible

Lab 15

neisseria

Lab 16

neisseria

Associated with PMNs

• Intracellular ) )pathogenic Neisseria

• Neisseria (diplococci )appear in electron microscope

Gram stain of N.gonorrhoea

Growth of N. gonorrhea in the media (Thayer-Martin agar )

Gram stain of Neisseria meningitis

Lab-16

Corynebacterium spp

Corynebacterium diphtheria

Loffler slope enriched media

blood tellurite medium

TOXIGENICITYIn vivo

in vitro(Elek’s test)

+ve control

• Unknown

Lab 17

Lactobacilli

• Gram positive lactobacilli

• Lactobacilli under electron microscope

Tomato juice agar (pH 5)

• Tomato juice agar

Rogosa agar

• Rogosa agar

Counting of streptococcus mutans and lactobacillus species in the saliva

Snyder test

• Snyder test

Lab 18

Enterobacteriaceae

Escherichia coli

Culture

1-Mac Conkey agar : Pink
colonies
due to the lactose fermentation
2-Blood agar : Some strains
show beta haemolysis


3-Eosin methylene blue
agar ( EMB ) : green to black
with metallic sheen.

Klebisella Spp.

Klebisella Spp.

Culture :

Blood agar : mucoid colonies .
MacConkey agar : large mucoid, pink colonies ( mucoid because accumulation of capsule ) .

Salmonella spp.

Culture :

Mac Conkey agar :
Colonies colourless ( NLF )

Salmonella – Shigella agar : brown colonies

with black center ( SS agar ) .

Shigella spp

Culture

MacConkey agar : Pale colonies except Sh. sonnei after 48hr .
SS agar : Colourless
without black center .

5. Proteus spp.

swarming
present only in the absence
of bile salt , .

Culture

1-nutrient agars produce swarming, fishy odor
2-urea agar.

Citrobacter :

Citrobacter :

Enterobacter

EMB PLATE: E. coli is seen on the left and E. aerogenes on the right

8.Serratia spp

Lab 19

mycobacterium

• Mycobacteria

Sputum testing

• Result:

• Acid fast bacteria appear as red
• Non-acid fast bacteria appear as blue


Culture
Lowenstein-Jensen medium: special selective media

Lowenstein-Jensen medium: special selective media

(rough, tough, and buff).

Chest x-ray

Tuberculin skin test (Mantoux skin test):

Tuberculin skin test (Mantoux skin test):

NIACIN TEST

+ve = yellow

Lab 20

Normal Flora

Genus Streptococcus

Genus Stomatococcus

Genus Actinomyces

Genus Lactobacillus

Genus Propionibacterium

Genus Veillonella

Genus Haemophilus

Genus Fusobacterium

Genus Treponema

Throat Culture

With my love